mrfp origene Search Results


pcmv6 ac mrfp  (OriGene)
93
OriGene pcmv6 ac mrfp
Pcmv6 Ac Mrfp, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+origene/pCMV6-AC-mRFP+Mammalian+Expression+Vector/pm25539916-65-2-3
Average 93 stars, based on 1 article reviews
pcmv6 ac mrfp - by Bioz Stars, 2026-10
93/100 stars
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plenti c mrfp p2a puro lenti vector  (OriGene)
91
OriGene plenti c mrfp p2a puro lenti vector
Plenti C Mrfp P2a Puro Lenti Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+origene/pLenti-mRFP-P2A-Puro+Lentiviral+Gene+Expression+Vector/10__1158_slash_2159___8290__cd___23___0601-198-10-12
Average 91 stars, based on 1 article reviews
plenti c mrfp p2a puro lenti vector - by Bioz Stars, 2026-10
91/100 stars
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anti mrfp  (OriGene)
90
OriGene anti mrfp
Anti Mrfp, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+origene/Mouse+monoclonal+mRFP+antibody%2C+clone+OTI3D7/pmc09054023-164-20-21
Average 90 stars, based on 1 article reviews
anti mrfp - by Bioz Stars, 2026-10
90/100 stars
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plasmid encoding tlr7 rfp  (OriGene)
93
OriGene plasmid encoding tlr7 rfp
FIGURE 3 | <t>TLR7</t> is involved in Tat-induced inflammatory responses in human astrocytes. (A–E) Activation of TLR7 by R837 (0–10 μg/mL for 48 h) significantly increased levels of galectin-3 (A), cathepsin B (B), IL-6 (C), IL-8 (D), and CCL2 (E) in the media of human astrocytes (n = 3). (F-J) Blocking TLR7 with CU-CPT9a (100 nM for 48 h) alone did not affect the release of inflammatory factors; However, CU-CPT9a significantly attenu- ated Tat (100 nM for 48 h)-induced increased levels of galectin-3 (F, n = 3), cathepsin B (G, n = 3), IL-6 (H, n = 6), IL-8 (I, n = 5), and CCL2 (J, n = 6) in the media of human astrocytes. Statistics: N = Independent replicates. One-way ANOVA with Tukey's post hoc test or two-way ANOVA with Tukey's post hoc test.
Plasmid Encoding Tlr7 Rfp, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+origene/pCMV6-AN-mRFP+Mammalian+Expression+Vector/pm40304459-219-13-17
Average 93 stars, based on 1 article reviews
plasmid encoding tlr7 rfp - by Bioz Stars, 2026-10
93/100 stars
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puromycin resistance gene  (OriGene)
90
OriGene puromycin resistance gene
FIGURE 3 | <t>TLR7</t> is involved in Tat-induced inflammatory responses in human astrocytes. (A–E) Activation of TLR7 by R837 (0–10 μg/mL for 48 h) significantly increased levels of galectin-3 (A), cathepsin B (B), IL-6 (C), IL-8 (D), and CCL2 (E) in the media of human astrocytes (n = 3). (F-J) Blocking TLR7 with CU-CPT9a (100 nM for 48 h) alone did not affect the release of inflammatory factors; However, CU-CPT9a significantly attenu- ated Tat (100 nM for 48 h)-induced increased levels of galectin-3 (F, n = 3), cathepsin B (G, n = 3), IL-6 (H, n = 6), IL-8 (I, n = 5), and CCL2 (J, n = 6) in the media of human astrocytes. Statistics: N = Independent replicates. One-way ANOVA with Tukey's post hoc test or two-way ANOVA with Tukey's post hoc test.
Puromycin Resistance Gene, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+origene/pAAVS1-mRFP-Puro-DNR/ppr0498691-144-29-32
Average 90 stars, based on 1 article reviews
puromycin resistance gene - by Bioz Stars, 2026-10
90/100 stars
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blasticidin resistant cassette  (OriGene)
93
OriGene blasticidin resistant cassette
FIGURE 3 | <t>TLR7</t> is involved in Tat-induced inflammatory responses in human astrocytes. (A–E) Activation of TLR7 by R837 (0–10 μg/mL for 48 h) significantly increased levels of galectin-3 (A), cathepsin B (B), IL-6 (C), IL-8 (D), and CCL2 (E) in the media of human astrocytes (n = 3). (F-J) Blocking TLR7 with CU-CPT9a (100 nM for 48 h) alone did not affect the release of inflammatory factors; However, CU-CPT9a significantly attenu- ated Tat (100 nM for 48 h)-induced increased levels of galectin-3 (F, n = 3), cathepsin B (G, n = 3), IL-6 (H, n = 6), IL-8 (I, n = 5), and CCL2 (J, n = 6) in the media of human astrocytes. Statistics: N = Independent replicates. One-way ANOVA with Tukey's post hoc test or two-way ANOVA with Tukey's post hoc test.
Blasticidin Resistant Cassette, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+origene/pAAVS1-mRFP-BSD-DNR/pmc12774452-323-11-17
Average 93 stars, based on 1 article reviews
blasticidin resistant cassette - by Bioz Stars, 2026-10
93/100 stars
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mouse α rfp  (OriGene)
91
OriGene mouse α rfp
FIGURE 3 | <t>TLR7</t> is involved in Tat-induced inflammatory responses in human astrocytes. (A–E) Activation of TLR7 by R837 (0–10 μg/mL for 48 h) significantly increased levels of galectin-3 (A), cathepsin B (B), IL-6 (C), IL-8 (D), and CCL2 (E) in the media of human astrocytes (n = 3). (F-J) Blocking TLR7 with CU-CPT9a (100 nM for 48 h) alone did not affect the release of inflammatory factors; However, CU-CPT9a significantly attenu- ated Tat (100 nM for 48 h)-induced increased levels of galectin-3 (F, n = 3), cathepsin B (G, n = 3), IL-6 (H, n = 6), IL-8 (I, n = 5), and CCL2 (J, n = 6) in the media of human astrocytes. Statistics: N = Independent replicates. One-way ANOVA with Tukey's post hoc test or two-way ANOVA with Tukey's post hoc test.
Mouse α Rfp, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrfp+origene/Carrier-free+(BSA%2Fglycerol-free)+Mouse+monoclonal+mRFP+antibody%2C+clone+OTI3D7/bio_rxiv__2023__08__31__555770-171-20-22
Average 91 stars, based on 1 article reviews
mouse α rfp - by Bioz Stars, 2026-10
91/100 stars
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Image Search Results


FIGURE 3 | TLR7 is involved in Tat-induced inflammatory responses in human astrocytes. (A–E) Activation of TLR7 by R837 (0–10 μg/mL for 48 h) significantly increased levels of galectin-3 (A), cathepsin B (B), IL-6 (C), IL-8 (D), and CCL2 (E) in the media of human astrocytes (n = 3). (F-J) Blocking TLR7 with CU-CPT9a (100 nM for 48 h) alone did not affect the release of inflammatory factors; However, CU-CPT9a significantly attenu- ated Tat (100 nM for 48 h)-induced increased levels of galectin-3 (F, n = 3), cathepsin B (G, n = 3), IL-6 (H, n = 6), IL-8 (I, n = 5), and CCL2 (J, n = 6) in the media of human astrocytes. Statistics: N = Independent replicates. One-way ANOVA with Tukey's post hoc test or two-way ANOVA with Tukey's post hoc test.

Journal: Aging cell

Article Title: TLR7 Mediates HIV-1 Tat-Induced Cellular Senescence in Human Astrocytes.

doi: 10.1111/acel.70086

Figure Lengend Snippet: FIGURE 3 | TLR7 is involved in Tat-induced inflammatory responses in human astrocytes. (A–E) Activation of TLR7 by R837 (0–10 μg/mL for 48 h) significantly increased levels of galectin-3 (A), cathepsin B (B), IL-6 (C), IL-8 (D), and CCL2 (E) in the media of human astrocytes (n = 3). (F-J) Blocking TLR7 with CU-CPT9a (100 nM for 48 h) alone did not affect the release of inflammatory factors; However, CU-CPT9a significantly attenu- ated Tat (100 nM for 48 h)-induced increased levels of galectin-3 (F, n = 3), cathepsin B (G, n = 3), IL-6 (H, n = 6), IL-8 (I, n = 5), and CCL2 (J, n = 6) in the media of human astrocytes. Statistics: N = Independent replicates. One-way ANOVA with Tukey's post hoc test or two-way ANOVA with Tukey's post hoc test.

Article Snippet: For co- localization of Tat with TLR7, human astrocytes were transfected with a plasmid encoding TLR7- RFP (Origene, cat#PS100049).

Techniques: Activation Assay, Blocking Assay

FIGURE 4 | TLR7 knockdown attenuates Tat-induced inflammatory response in human astrocytes. (A) Quantitative immunoblotting data showed that TLR7 was knocked down with specific siRNAs in human astrocytes (n = 3). (B-F) TLR7 knockdown alone did not affect the release of these inflammatory factors; However, TLR7 knockdown significantly attenuated Tat-induced increases in IL-6 (B, n = 5), IL-8 (C, n = 4), CCL2 (E, n = 5), galectin-3 (E, n = 4), and cathepsin B (F, n = 3) in the media of cultured human astrocytes. Statistics: N = Independent replicates. Two-way ANOVA with Tukey's post hoc test and two tailed student's t-test.

Journal: Aging cell

Article Title: TLR7 Mediates HIV-1 Tat-Induced Cellular Senescence in Human Astrocytes.

doi: 10.1111/acel.70086

Figure Lengend Snippet: FIGURE 4 | TLR7 knockdown attenuates Tat-induced inflammatory response in human astrocytes. (A) Quantitative immunoblotting data showed that TLR7 was knocked down with specific siRNAs in human astrocytes (n = 3). (B-F) TLR7 knockdown alone did not affect the release of these inflammatory factors; However, TLR7 knockdown significantly attenuated Tat-induced increases in IL-6 (B, n = 5), IL-8 (C, n = 4), CCL2 (E, n = 5), galectin-3 (E, n = 4), and cathepsin B (F, n = 3) in the media of cultured human astrocytes. Statistics: N = Independent replicates. Two-way ANOVA with Tukey's post hoc test and two tailed student's t-test.

Article Snippet: For co- localization of Tat with TLR7, human astrocytes were transfected with a plasmid encoding TLR7- RFP (Origene, cat#PS100049).

Techniques: Knockdown, Western Blot, Cell Culture, Two Tailed Test

FIGURE 6 | TLR7 activation induces a senescence-like phenotype in human astrocytes. (A) TLR7 agonist (R837) (10 μg/mL for 48 h) significantly decreased proliferation, as indicated by BrdU incorporation, in human astrocytes (n = 3). (B, C) TLR7 activation with R837 (10 μg/mL for 48 h) sig- nificantly increased protein levels of senescence markers p16Ink4a (B, n = 3 Independent replicates) and p21CIP1 (C, n = 5) in human astrocytes. (D, E) TLR7 agonist R837 (10 μg/mL) significantly increased the percentage of SA-β-gal positive cells at 48 h (D) and 72 h (E) post-treatment (n = 3). (F, G) TLR7 agonist R837 (10 μg/mL), significantly increased SA-β-gal activity at 48 h (F) and 72 h (G) post-treatment (n = 4). Statistics: N = Independent replicates. One-way ANOVA followed by Tukey's post hoc test and two tailed student's t-test.

Journal: Aging cell

Article Title: TLR7 Mediates HIV-1 Tat-Induced Cellular Senescence in Human Astrocytes.

doi: 10.1111/acel.70086

Figure Lengend Snippet: FIGURE 6 | TLR7 activation induces a senescence-like phenotype in human astrocytes. (A) TLR7 agonist (R837) (10 μg/mL for 48 h) significantly decreased proliferation, as indicated by BrdU incorporation, in human astrocytes (n = 3). (B, C) TLR7 activation with R837 (10 μg/mL for 48 h) sig- nificantly increased protein levels of senescence markers p16Ink4a (B, n = 3 Independent replicates) and p21CIP1 (C, n = 5) in human astrocytes. (D, E) TLR7 agonist R837 (10 μg/mL) significantly increased the percentage of SA-β-gal positive cells at 48 h (D) and 72 h (E) post-treatment (n = 3). (F, G) TLR7 agonist R837 (10 μg/mL), significantly increased SA-β-gal activity at 48 h (F) and 72 h (G) post-treatment (n = 4). Statistics: N = Independent replicates. One-way ANOVA followed by Tukey's post hoc test and two tailed student's t-test.

Article Snippet: For co- localization of Tat with TLR7, human astrocytes were transfected with a plasmid encoding TLR7- RFP (Origene, cat#PS100049).

Techniques: Activation Assay, BrdU Incorporation Assay, Activity Assay, Two Tailed Test

FIGURE 7 | TLR7 knockdown attenuates HIV-1 Tat-induced senescence-like phenotype in human astrocytes. (A) TLR7 knockdown signifi- cantly attenuated Tat-induced increases in p16Ink4a protein levels in human astrocytes (n = 4). (B, C) TLR7 knockdown significantly attenuated Tat (100 nM)-induced increases in SA-β-gal activity at 48 h (B, n = 4) and 72 h (C, n = 3) post-treatment. Statistics: N = Independent replicates. Two-way ANOVA followed by Tukey's post hoc test and two tailed student's t-test.

Journal: Aging cell

Article Title: TLR7 Mediates HIV-1 Tat-Induced Cellular Senescence in Human Astrocytes.

doi: 10.1111/acel.70086

Figure Lengend Snippet: FIGURE 7 | TLR7 knockdown attenuates HIV-1 Tat-induced senescence-like phenotype in human astrocytes. (A) TLR7 knockdown signifi- cantly attenuated Tat-induced increases in p16Ink4a protein levels in human astrocytes (n = 4). (B, C) TLR7 knockdown significantly attenuated Tat (100 nM)-induced increases in SA-β-gal activity at 48 h (B, n = 4) and 72 h (C, n = 3) post-treatment. Statistics: N = Independent replicates. Two-way ANOVA followed by Tukey's post hoc test and two tailed student's t-test.

Article Snippet: For co- localization of Tat with TLR7, human astrocytes were transfected with a plasmid encoding TLR7- RFP (Origene, cat#PS100049).

Techniques: Knockdown, Activity Assay, Two Tailed Test